Por favor, use este identificador para citar o enlazar este ítem: http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/125
Optimization of growth regulators in organogenesis of Bletia purpurea (Lam.) using response surface design and genetic evaluation
JULIA ESTHER YAH CHULIM
MARIA DE LOURDES REYES ESCOGIDO
GUADALUPE LOPEZ PUC
INGRID MAYANIN RODRIGUEZ BUENFIL
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.5897/AJB12.1028
DOI: 10.5897/AJB12.1028
This paper reports the optimal concentrations of indol acetic acid (IAA) and benzylaminopurine to stimulate morphogenetic induction Bletia purpurea (Lam.) and the application of random amplification of polymorphic deoxyribonucleic acid (DNA) (RAPD) for genetic evaluation of micropropagated plantlets. Organogenesis was induced from B. purpurea (Lam.) explant pseudobulbs in Murashige and Skoog (MS) medium at full ionic strength and supplemented with 20 g/l sucrose, 2 g/l activated carbon and 2.2 g/l Gel Rite. Optimum growth regulator concentrations were determined by response surface design, according to Box-Benhken. At 15 days after morphogenic induction was observed, direct shoots formation on the explant was attained, and optimum concentrations for the greatest number of shoots was 1.8 mg/l Indol acetic acid and no benzylaminopurine. Comparison of RAPD genetic profiles for mother plantlets and regenerated daughter plantlets showed no changes in the genome based on the oligonucleotides used. The conditions applied in this protocol produces plantlets which maintain the genetic traits of the first generation.
African Journal of Biotechnology
2012
Artículo
Inglés
Beneficiarios de fondos federales y solicitantes
Bibliotecarios
Estudiantes
Investigadores
Maestros
Esther, Y. C. J., Mayanin, R. B. I., de Lourdes, R. E. M., & Guadalupe, L. P. (2012). Optimization of growth regulators in organogenesis of Bletia purpurea (Lam.) using response surface design and genetic evaluation. African Journal of Biotechnology, 11(57), 12045.
CIENCIAS DE LA VIDA
Aparece en las colecciones: ARTICULOS

Cargar archivos:


Fichero Descripción Tamaño Formato  
Optimization of growth regulators. Yah chulim et al. Lopez Puc.pdf425.43 kBAdobe PDFVisualizar/Abrir