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http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/103| AN EFFECTIVE IN VITRO SLOW GROWTH PROTOCOL FOR CONSERVATION OF THE ORCHID Epidendrum chlorocorymbos SCHLTR. | |
| Guadalupe Lopez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| in vitro storage; mannitol; sorbitol; Epidendrum chlorocorymbos Schltr. | |
| Efficient slow growth protocol for the orchid E. chlorocorymbos Schltr. was developed using in vitro conservation studies. Seedlings were placed in MS culture medium and a 2x3x3 factorial design applied to evaluate the effects of supplementing the medium with mannitol (0, 1, 2 and 3%), sucrose (0, 1, 2 and 3%) or sorbitol (0, 1, 2 and 3%). Experimental conditions were a 16:8 h photoperiod, 23 ± 2 °C temperature and 50-80% relative humidity. At 6 months, the best treatment was MS medium at half ionic strength with 1% sorbitol. The culture was also free of contamination. This resulted in slow growth and normal morphology during maintenance and successful growth afterwards. Shoots were subsequently recovered, multiplied and rooted on MS medium with sucrose 3% without addition of growth regulators. | |
| Tropical and Subtropical Agroecosystems | |
| 2013 | |
| Artículo | |
| Inglés | |
| Beneficiarios de fondos federales y solicitantes Bibliotecarios Empresas Estudiantes Investigadores | |
| Lopez-Puc, G. (2013). AN EFFECTIVE IN VITRO SLOW GROWTH PROTOCOL FOR CONSERVATION OF THE ORCHID Epidendrum chlorocorymbos SCHLTR. Tropical and Subtropical Agroecosystems, 16(1) | |
| BIOLOGÍA Y QUÍMICA | |
| Aparece en las colecciones: | ARTICULOS |
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| An effective. Lopez Puc.pdf | 409.53 kB | Adobe PDF | Visualizar/Abrir |