Please use this identifier to cite or link to this item: http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/155
High-throughput screening method for alkaline lipases using triolein as substrate
Jorge Alberto Rodriguez Gonzalez
MARIA DE LOS ANGELES CAMACHO RUIZ
JUAN CARLOS MATEOS DIAZ
ROSA MARIA CAMACHO RUIZ
Acceso Abierto
Atribución-NoComercial-SinDerivadas
lipase, triacylglycerol, microtiter plate
Lipases (EC 3.1.1.3) are soluble enzymes that can act on natural insoluble triacylglycerols (TAG) (1). They are present in many bacteria, fungi, plants, and animals, and have been used in numerous applications such as food, cosmetics, detergents, and pharmaceutical industries (2). Most lipases have been reported to be optimally active at neutral pH (3), but it will be useful to have alkaline lipases for biocatalysis and industrial applications. The success of their screening depends on reliable, appropriate, and sensitive assays to detect a true lipase activity. High-throughput screening (HTS) methods for lipases are usually performed using non appropriate synthetic chromogenic substrates (4); new HTS methods employing natural substrates of lipases are necessary. In this work we propose a HTS method for alkaline lipases using triolein (an insoluble TAG), wherein the lipolytic activity can be measured spectrophotometrically due to the fatty acid liberation and the color change of a pH indicator.
Sociedad Mexicana de Biotecnología y Bioingeniería
2013
Póster de congreso
Inglés
Estudiantes
Investigadores
Maestros
BIOLOGÍA Y QUÍMICA
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