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http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/155| High-throughput screening method for alkaline lipases using triolein as substrate | |
| Jorge Alberto Rodriguez Gonzalez | |
| MARIA DE LOS ANGELES CAMACHO RUIZ JUAN CARLOS MATEOS DIAZ ROSA MARIA CAMACHO RUIZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| lipase, triacylglycerol, microtiter plate | |
| Lipases (EC 3.1.1.3) are soluble enzymes that can act on natural insoluble triacylglycerols (TAG) (1). They are present in many bacteria, fungi, plants, and animals, and have been used in numerous applications such as food, cosmetics, detergents, and pharmaceutical industries (2). Most lipases have been reported to be optimally active at neutral pH (3), but it will be useful to have alkaline lipases for biocatalysis and industrial applications. The success of their screening depends on reliable, appropriate, and sensitive assays to detect a true lipase activity. High-throughput screening (HTS) methods for lipases are usually performed using non appropriate synthetic chromogenic substrates (4); new HTS methods employing natural substrates of lipases are necessary. In this work we propose a HTS method for alkaline lipases using triolein (an insoluble TAG), wherein the lipolytic activity can be measured spectrophotometrically due to the fatty acid liberation and the color change of a pH indicator. | |
| Sociedad Mexicana de Biotecnología y Bioingeniería | |
| 2013 | |
| Póster de congreso | |
| Inglés | |
| Estudiantes Investigadores Maestros | |
| BIOLOGÍA Y QUÍMICA | |
| Aparece en las colecciones: | CARTEL / POSTER |
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| Cartel Angeles 2013.pdf | Cartel | 193.59 kB | Adobe PDF | Visualizar/Abrir |