Please use this identifier to cite or link to this item: http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/187
Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate
Enrique J Herrera López
JORGE ENRIQUE PLIEGO SANDOVAL
JUAN CARLOS MATEOS DIAZ
JORGE ALBERTO RODRIGUEZ GONZALEZ
ALEJANDRO RICARDO FEMAT FLORES
GEORGINA CORAL SANDOVAL FABIAN
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.3390/s150202798
monitoring; lipase/esterase activity; sequential injection analysis; stopped flow; p-nitrophenyl esters
Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05–1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed
SENSORS
2015-01
Artículo
Inglés
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Pliego, J., Mateos, J. C., Rodriguez, J., Valero, F., Baeza, M., Femat, R., ... & Herrera-López, E. J. (2015). Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors, 15(2), 2798-2811.
INGENIERÍA Y TECNOLOGÍA
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