Please use this identifier to cite or link to this item: http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/397
Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein
CARLOS ALBERTO MANUEL CABRERA
ALBA ADRIANA VALLEJO CARDONA
EDUARDO PADILLA CAMBEROS
RODOLFO HERNANDEZ GUTIERREZ
SARA ELISA HERRERA RODRIGUEZ
ABEL GUTIERREZ ORTEGA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.1186/s12985-016-0651-y
virus del grabado del tabaco, proteína de la cápside, partículas pseudovíricas, etiqueta de hexahistidinas, adyuvante, proteína quimérica
"Abstract Background: Assembly of recombinant capsid proteins into virus-like particles (VLPs) still represents an interesting challenge in virus-based nanotechnologies. The structure of VLPs has gained importance for the development and design of new adjuvants and antigen carriers. The potential of Tobacco etch virus capsid protein (TEV CP) as adjuvant has not been evaluated to date. Findings: Two constructs for TEV CP expression in Escherichia coli were generated: a wild-type version (TEV-CP) and a C-terminal hexahistidine (His)-tagged version (His-TEV-CP). Although both versions were expressed in the soluble fraction of E. coli lysates, only His-TEV-CP self-assembled into micrometric flexuous filamentous VLPs. In addition, the His-tag enabled high yields and facilitated purification of TEV VLPs. These TEV VLPs elicited broader IgG2- specific antibody response against a novel porcine reproductive and respiratory syndrome virus (PRRSV) protein when compared to the potent IgG1 response induced by the protein alone. Conclusions: His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs, some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against a soluble antigen".
BioMed Central
29-11-2016
Artículo
Virology Journal
Inglés
Estudiantes
Investigadores
BIOLOGÍA MOLECULAR
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:ARTICULOS

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