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http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/397| Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein | |
| CARLOS ALBERTO MANUEL CABRERA ALBA ADRIANA VALLEJO CARDONA EDUARDO PADILLA CAMBEROS RODOLFO HERNANDEZ GUTIERREZ SARA ELISA HERRERA RODRIGUEZ ABEL GUTIERREZ ORTEGA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1186/s12985-016-0651-y | |
| virus del grabado del tabaco, proteína de la cápside, partículas pseudovíricas, etiqueta de hexahistidinas, adyuvante, proteína quimérica | |
| "Abstract Background: Assembly of recombinant capsid proteins into virus-like particles (VLPs) still represents an interesting challenge in virus-based nanotechnologies. The structure of VLPs has gained importance for the development and design of new adjuvants and antigen carriers. The potential of Tobacco etch virus capsid protein (TEV CP) as adjuvant has not been evaluated to date. Findings: Two constructs for TEV CP expression in Escherichia coli were generated: a wild-type version (TEV-CP) and a C-terminal hexahistidine (His)-tagged version (His-TEV-CP). Although both versions were expressed in the soluble fraction of E. coli lysates, only His-TEV-CP self-assembled into micrometric flexuous filamentous VLPs. In addition, the His-tag enabled high yields and facilitated purification of TEV VLPs. These TEV VLPs elicited broader IgG2- specific antibody response against a novel porcine reproductive and respiratory syndrome virus (PRRSV) protein when compared to the potent IgG1 response induced by the protein alone. Conclusions: His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs, some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against a soluble antigen". | |
| BioMed Central | |
| 29-11-2016 | |
| Artículo | |
| Virology Journal | |
| Inglés | |
| Estudiantes Investigadores | |
| BIOLOGÍA MOLECULAR | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | ARTICULOS |
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| Self-assembly of hexahistidine.pdf | 706.21 kB | Adobe PDF | Visualizar/Abrir |