Por favor, use este identificador para citar o enlazar este ítem:
http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/484| Development of a mucoadhesive buccal film as a carrier of Lippia graveolens oil emulsions and its in vitro evaluation against Candida albicans | |
| ROSA JARUMY LOPEZ RIVERA | |
| HUGO ESPINOSA ANDREWS | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| The purpose of this work is to develop a mucoadhesive film that serves as a carrier for Lippia graveolens essential oil (LGO) emulsion and to evaluate in vitro their inhibitory effect against opportunistic pathogen Candida. albicans. For this, different ratio Chitosan: Pectin (100:0, 75:25, 50:50, 25:75, 0:100) films were obtained by casting technique with different concentration of glycerol as a plasticizer (0, 10 and 30%). Water-uptake, erosion, and mucoadhesion were evaluated. Films with CH:P ratio of 100:0 with 30% glycerol, 75:25 with 10% glycerol and 50:50 with 10% glycerol showed better mucoadhesion and physical properties. Characterization of LGO was carried out, and major components thymol and carvacrol were quantified by GC-MS, these phenols were present in a 2:1 ratio. Three different emulsions with LGO were developed and characterized, their inhibitory effect was evaluated against C. albicans. Arabic gum (GA), Hydroxylated lecithin (LecH) and polysorbate 80 (T80) were used as emulsifiers. The particle size of emulsions was 1597±11.1, 179±5.5 and 137.3±4.2 respectively. All emulsions had an inhibitory effect against C. albicans. Volumes of emulsions that inhibit the 99% of C. albicans were used for the in vitro test of films together with emulsion. With this work it was possible to develop three different emulsions with an inhibitory effect against C. albicans, likewise, these emulsions showed an inhibitory effect in a localized way when evaluated together with the mucoadhesive films against C. albicans | |
| 12-11-2017 | |
| Tesis de maestría | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Aparece en las colecciones: | MAESTRÍA EN CIENCIAS EN INNOVACIÓN BIOTECNOLÓGICA |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Rosa Jarumy Rivera López.pdf | 1.86 MB | Adobe PDF | Visualizar/Abrir |