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Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi
Jorge Verdín
EDDY FRANCISCO SANCHEZ LEON HING
ROSA AURELIA FAJARDO SOMERA
CARLOS ALBERTO LEAL MORALES
SALOMON BARTNICKI GARCIA
MERITXELL RIQUELME PEREZ
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.21769/BioProtoc.1611
" Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging techniques, which make possible to relate a single protein with, for example, a discrete population of intracellular vesicles and their in vivo dynamics (Verdín et al., 2009; Fajardo-Somera et al., 2013; Sánchez-León et al., 2015). Here, we describe the density gradient centrifugation and fractionation analysis of cell-free homogenates of a Neurospora crassa (N. crassa) strain that expresses CHS-6 chitin synthase fused to the green fluorescent protein (Riquelme et al., 2007). "
Bio-protocol
05-10-2015
Artículo
bio-protocol vol 5, Iss 19, Oct 05, 2015
Inglés
BIOLOGÍA Y QUÍMICA
Versión aceptada
acceptedVersion - Versión aceptada
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