Por favor, use este identificador para citar o enlazar este ítem:
http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/614| Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi | |
| Jorge Verdín EDDY FRANCISCO SANCHEZ LEON HING ROSA AURELIA FAJARDO SOMERA CARLOS ALBERTO LEAL MORALES SALOMON BARTNICKI GARCIA MERITXELL RIQUELME PEREZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.21769/BioProtoc.1611 | |
| " Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging techniques, which make possible to relate a single protein with, for example, a discrete population of intracellular vesicles and their in vivo dynamics (Verdín et al., 2009; Fajardo-Somera et al., 2013; Sánchez-León et al., 2015). Here, we describe the density gradient centrifugation and fractionation analysis of cell-free homogenates of a Neurospora crassa (N. crassa) strain that expresses CHS-6 chitin synthase fused to the green fluorescent protein (Riquelme et al., 2007). " | |
| Bio-protocol | |
| 05-10-2015 | |
| Artículo | |
| bio-protocol vol 5, Iss 19, Oct 05, 2015 | |
| Inglés | |
| BIOLOGÍA Y QUÍMICA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | ARTICULOS |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Bio-protocol1611.pdf | 817.32 kB | Adobe PDF | Visualizar/Abrir |