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Comparative analysis of 16s ribosomal RNA of "Candidatus liberibacter asiaticus" associated with Huanglongbing diseases of persian lime and Mexican lime reveals a major haplotype with worldwide distribution | |
ANGELICA MORENO ENRIQUEZ YERENI MINERO GARCIA Jorge Humberto Ramírez Prado Emiliano Loeza Kuk Alberto Uc Várguez OSCAR ALBERTO MORENO VALENZUELA | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
10.5897/AJMR2014.6706 | |
"Huanglongbing (HLB) is considered as one of the mos destructive and devastating disease of citrus trees worldwide, In the present study, we analyzed the genetic diversity of the 16 S rDNA gene sequence of ´Candidatus Liberibacter asiaticus´(´Ca.L. asiaticus´) strains from symptomatic trees of Cirus GenBank database. Two hundred and fourteen (214) sample4s of trees collected in the Peninsula were analyzed using PCR, although the positive trees were increasedto seventy by real-time q PCR. Blast searches sequences of ´Ca.L. asiaticus´. Multiple alignment showed 2 clusters of sequences, wherein the first group were 100% identical, and the second group was characterized bythe presence of polymorphic H36PENINSULAR. Analysis of the 16S rDNA sequences from GenBank revealed the same behavior, wherein one group exhibited 100% similarity (within the group). Alignment of the first groups from each suggest that sequence H36PENINSULAR be considered the principal haplotype of the 16S rRNA gene of ´Ca.L. asiaticus´beacuse it exhibits worldwide distribution and dominance." | |
African Journal of Microbiology Research | |
13-06-2014 | |
Artículo | |
African Journal of Microbiology Research Vol. 8(30), pp. 2861-2873 | |
Inglés | |
BIOLOGÍA Y QUÍMICA | |
Versión aceptada | |
acceptedVersion - Versión aceptada | |
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