Please use this identifier to cite or link to this item: http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/625
Comparative analysis of 16s ribosomal RNA of "Candidatus liberibacter asiaticus" associated with Huanglongbing diseases of persian lime and Mexican lime reveals a major haplotype with worldwide distribution
ANGELICA MORENO ENRIQUEZ
YERENI MINERO GARCIA
Jorge Humberto Ramírez Prado
Emiliano Loeza Kuk
Alberto Uc Várguez
OSCAR ALBERTO MORENO VALENZUELA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.5897/AJMR2014.6706
"Huanglongbing (HLB) is considered as one of the mos destructive and devastating disease of citrus trees worldwide, In the present study, we analyzed the genetic diversity of the 16 S rDNA gene sequence of ´Candidatus Liberibacter asiaticus´(´Ca.L. asiaticus´) strains from symptomatic trees of Cirus GenBank database. Two hundred and fourteen (214) sample4s of trees collected in the Peninsula were analyzed using PCR, although the positive trees were increasedto seventy by real-time q PCR. Blast searches sequences of ´Ca.L. asiaticus´. Multiple alignment showed 2 clusters of sequences, wherein the first group were 100% identical, and the second group was characterized bythe presence of polymorphic H36PENINSULAR. Analysis of the 16S rDNA sequences from GenBank revealed the same behavior, wherein one group exhibited 100% similarity (within the group). Alignment of the first groups from each suggest that sequence H36PENINSULAR be considered the principal haplotype of the 16S rRNA gene of ´Ca.L. asiaticus´beacuse it exhibits worldwide distribution and dominance."
African Journal of Microbiology Research
13-06-2014
Artículo
African Journal of Microbiology Research Vol. 8(30), pp. 2861-2873
Inglés
BIOLOGÍA Y QUÍMICA
Versión aceptada
acceptedVersion - Versión aceptada
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