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http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/752| Type C feruloyl esterase from Aspergillus ochraceus: A selective biocatalyst for the synthesis of butyl hydroxycinnamates in a ternary solvent system | |
| EVELYN ROMERO BORBON DANIEL ALBERTO GRAJALES HERNANDEZ MARIANA ANTONIETA ARMENDARIZ RUIZ Lorena Ramírez Velasco Jorge Alberto Rodríguez González LUIS ALBERTO CIRA CHAVEZ MARIA ISABEL ESTRADA ALVARADO JUAN CARLOS MATEOS DIAZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.ejbt.2018.06.004 | |
| "Background Aspergillus ochraceus was isolated from coffee pulp and selected as an interesting hydroxycinnamoyl esterase strain producer, using an activity microplate high-throughput screening method. In this work, we purified and characterized a new type C A. ochraceus feruloyl esterase (AocFaeC), which synthesized specifically butyl hydroxycinnamates in a ternary solvent system. Results AocFaeC was produced by solid state fermentation, reaching its maximal activity (1.1 U/g) after 48 h of culture. After purification, the monomeric protein (34 kDa) showed a specific activity of 57.9 U/mg towards methyl ferulate. AocFaeC biochemical characterization confirmed its identity as a type C feruloyl esterase and suggested the presence of a catalytic serine in the active site. Its maximum hydrolytic activity was achieved at 40°C and pH 6.5 and increased by 109 and 77% with Ca2 + and Mg2 +, but decreased by 90 and 45% with Hg2 + and Cu2 +, respectively. The initial butyl ferulate synthesis rate increased from 0.8 to 23.7 nmol/min after transesterification condition improvement, using an isooctane:butanol:water ternary solvent system, surprisingly the synthesis activity using other alcohols was negligible. At these conditions, the synthesis specific activities for butyl p-coumarate, sinapinate, ferulate, and caffeate were 87.3, 97.6, 168.2, and 234 U/μmol, respectively. Remarkably, AocFaeC showed 5 folds higher butyl caffeate synthesis rate compared to type B Aspergillus niger feruloyl esterase, a well-known enzyme for its elevated activity towards caffeic acid esters. Conclusions Type C feruloyl esterase from A. ochraceus is a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system." | |
| Graciela Munoz | |
| 2018 | |
| Artículo | |
| Electronic Journal of Biotechnology, 2018, 35. | |
| Inglés | |
| Bibliotecarios Estudiantes Investigadores | |
| Romero E, Grajales D, Armendáriz M, et al. Type C feruloyl esterase from Aspergillus ochraceus: A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system. Electron J Biotechnol 2018;35. | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
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