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Biocontrol of soft rot agave by bacteriophages
GABRIEL RINCON ENRIQUEZ
Acceso Abierto
Atribución-NoComercial-SinDerivadas
ISBN: 978-607-96619-1-5
Bacterial viruses, Bacillus sp, Plant pathogenic bacteria
Cultivation of several species of the genus Agave is used as raw material for the production of beverages such as A. tequilana and A. cupreata. However, the cultivation of these species is threatened by pests and phytosanitary problems, among them are the weevil (Scyphophorus acupunctatus), wilt (Fusarium oxysporum) and soft rot (several bacterial species). Especially rot causes great losses in agave fields for the production of tequila and mezcal in the states of Jalisco and Michoacán in Mexico. Results of chemical control of soft rot have not been successful. On the other hand, bacterial viruses (bacteriophages) are employed for efficient fight off bacterial diseases in plants. So the aim of this work was to isolate and characterize bacteriophages associated with the causal agent of soft rot for A. tequilana and A. cupreata. Samples of crops with agave soft rot were performed on sites of Jalisco and Michoacán. Bacteriophages were isolated from phytopathogenic and virulent bacterial isolates and were characterized by transmission electron microscope and restriction patterns. Three morphotypes of bacteriophages were isolated from four bacterial species involved in soft rot of agave. The bacteriophages lysed differential magnitude the bacterial cultures in vitro. These results suggest the possible application for biocontrol of bacteria involved in soft rot of agave, and this would help to the sustainable production of the raw material for tequila and mezcal.
II International Symposium on Agave
22-12-2014
Memoria de congreso
II International Symposium on Agave
Inglés
Estudiantes
Investigadores
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
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