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Phytic acid quantification in agroindustrial residues and their potential as inducers for phytase production in solid state fermentation
Acceso Abierto
Phytic Acid, Agroindustrial Residues, Phytase
Phytases have been one of the focal enzymes for animal nutrition and human health during the past two decades. In order to study typical agroindustrial residues used in animal feed as potential inducers for phytase production in Solid State Fermentation (SSF), a methodology to quantify phytic acid in solids was implemented. The agroindustrial residues used for this study were: agave bagasse, sugarcane bagasse, coffe husk, corn husk, cottonseed meal, wheat straw and wheat bran. Bartlett (1959) technique for phytic acid extraction and Haug-Lantzsch (1983) for phytic acid quantification were implemented. Three different acids were assayed for phytic acid extraction (3% HCl, 5% H2SO4 and 10% trichloroacetic acid) using 1:20 (w/v) ratio for 1 h at 30°C. The acid showing the best extraction results was used for further studies to improve the phytic acid extraction conditions. Three acid concentrations (0.2M, 0.6M and 1M); temperatures (25°C, 50°C, and 100°C) and weigh/volume ratios (1:5, 1:10 and 1:20) were tested. Solid state fermentations were carried out for 8 days, pH 6.5, 60% moisture and 30°C, using polyurethane as inert support and a laboratory strain indentified as Aspergillus niger. Only, three agroindustrial residues (corn husk, cottonseed meal and wheat bran) showed a considerable phytic acid content (approximately 0.5%) using 3% HCl for extraction. The best conditions for extracting phytic acid were: 0.2M of HCl, 25°C and 1:20 ratio for the three agroindustrial residues, finding 0.57 %, 0.45 % and 0.67 % of phytic acid for corn husk, cottonseed meal and wheat bran, respectively. The three extracts obtained were assayed as inducers for phytase production in SSF. The maximum phytase activity reached was 0.39±0.09 U/gDM (grams of dry matter) at 24 h of fermentation using a wheat bran extract as inducer, followed by corn husk extract with 0.35±0.01 U/gDM at 24 h of fermentation; cottonseed meal extract used as SSF inducer, does not present significant phytase activity. It was possible to implement a quantification methodology for phytic acid in agroindustrial residues and the extracts obtained proved their potential as inducers in phytase production.
Asociación Mexicana de Ciencias de los Alimentos
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